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Molecular alterations and clinical relevance in esophageal squamous cell carcinoma
null
《医学前沿(英文)》 2013年 第7卷 第4期 页码 401-410 doi: 10.1007/s11684-013-0286-y
Esophageal squamous cell carcinoma (ESCC) is one of the most common types of gastrointestinal cancers, and the fourth leading cause of cancer-related deaths in China. Early detection and intervention in time may dramatically increase the survival of the patients by initiating treatment regimens during earlier stages of ESCC or even during precancerous stages. Molecular classification will be useful for subtyping esophageal tumors or precancerous lesions to improve current therapeutics or early intervention of the disease. In this review, we summarize the findings in investigating the molecular alterations and clinical relevance of ESCC.
关键词: esophageal squamous cell carcinoma (ESCC) early detection molecular classification molecular markers
Repurposed benzydamine targeting CDK2 suppresses the growth of esophageal squamous cell carcinoma
《医学前沿(英文)》 2023年 第17卷 第2期 页码 290-303 doi: 10.1007/s11684-022-0956-8
关键词: benzydamine cyclin-dependent kinase 2 patient-derived xenograft esophageal squamous cell carcinoma
Relative expression of PTTG and bFGF in oral squamous cell carcinoma and Tca8113
Yumei DING BM , Lili CHEN MD , Bo CHENG PhD , Handong ZHANG MM ,
《医学前沿(英文)》 2009年 第3卷 第3期 页码 357-362 doi: 10.1007/s11684-009-0046-1
关键词: carcinoma squamous cell pituitary tumor transforming gene (PTTG) protein basic fibroblast growth factor
Junfang JI, Kun WU, Min WU, Qimin ZHAN,
《医学前沿(英文)》 2010年 第4卷 第4期 页码 412-418 doi: 10.1007/s11684-010-0260-x
Genomic variations in the counterpart normal controls of lung squamous cell carcinomas
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《医学前沿(英文)》 2018年 第12卷 第3期 页码 280-288 doi: 10.1007/s11684-017-0580-1
Lung squamous cell carcinoma (LUSC) causes approximately 400 000 deaths each year worldwide. The occurrence of LUSC is attributed to exposure to cigarette smoke, which induces the development of numerous genomic abnormalities. However, few studies have investigated the genomic variations that occur only in normal tissues that have been similarly exposed to tobacco smoke as tumor tissues. In this study, we sequenced the whole genomes of three normal lung tissue samples and their paired adjacent squamous cell carcinomas. We then called genomic variations specific to the normal lung tissues through filtering the genomic sequence of the normal lung tissues against that of the paired tumors, the reference human genome, the dbSNP138 common germline variants, and the variations derived from sequencing artifacts. To expand these observations, the whole exome sequences of 478 counterpart normal controls (CNCs) and paired LUSCs of The Cancer Genome Atlas (TCGA) dataset were analyzed. Sixteen genomic variations were called in the three normal lung tissues. These variations were confirmed by Sanger capillary sequencing. A mean of 0.5661 exonic variations/Mb and 7.7887 altered genes per sample were identified in the CNC genome sequences of TCGA. In these CNCs, C:G→T:A transitions, which are the genomic signatures of tobacco carcinogen N-methyl-N-nitro-N-nitrosoguanidine, were the predominant nucleotide changes. Twenty five genes in CNCs had a variation rate that exceeded 2%, including ARSD (18.62%), MUC4 (8.79%), and RBMX (7.11%). CNC variations in CTAGE5 and USP17L7 were associated with the poor prognosis of patients with LUSC. Our results uncovered previously unreported genomic variations in CNCs, rather than LUSCs, that may be involved in the development of LUSC.
关键词: lung cancer counterpart normal control genomic variations
《医学前沿(英文)》 2023年 第17卷 第1期 页码 119-131 doi: 10.1007/s11684-022-0949-7
关键词: GDF15 esophageal squamous cell carcinoma chemoresistance cellular metabolism TGFBR2 AKT
null
《医学前沿(英文)》 2015年 第9卷 第4期 页码 478-486 doi: 10.1007/s11684-015-0420-0
Telomeres play a critical role in biological ageing by maintaining chromosomal integrity and preventing chromosome ends fusion. Epidemiological studies have suggested that inter-individual differences of telomere length could affect predisposition to multiple cancers, but evidence regarding esophageal squamous cell carcinoma (ESCC) was still uncertain. Several telomere length-related single nucleotide polymorphisms (TL-SNPs) in Caucasians have been reported in genome-wide association studies. However, the effects of telomere length and TL-SNPs on ESCC development are unclear. Therefore, we conducted a case-control study (1045 ESCC cases and 1433 controls) to evaluate the associations between telomere length, TL-SNPs, and ESCC risk in Chinese population. As a result, ESCC cases showed overall shorter relative telomere length (RTL) (median: 1.34) than controls (median: 1.50, P<0.001). More interestingly, an evident nonlinear U-shaped association was observed between RTL and ESCC risk (P<0.001), with odds ratios (95% confidence interval) equal to 2.40 (1.84–3.14), 1.36 (1.03–1.79), 1.01 (0.76–1.35), and 1.37 (1.03–1.82) for individuals in the 1st (the shortest), 2nd, 3rd, and 5th (the longest) quintile, respectively, compared with those in the 4th quintile as reference group. No significant associations were observed between the eight reported TL-SNPs and ESCC susceptibility. These findings suggest that either short or extremely long telomeres may be risk factors for ESCC in the Chinese population.
关键词: esophageal squamous cell carcinoma telomere length genetic variants susceptibility genome-wide association study
《医学前沿(英文)》 2022年 第16卷 第4期 页码 610-617 doi: 10.1007/s11684-021-0827-8
关键词: bevacizumab elderly patient advanced non-small-cell lung cancer overall survival toxicity
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《医学前沿(英文)》 2017年 第11卷 第2期 页码 223-228 doi: 10.1007/s11684-017-0517-8
The objective of this study is to guide a triage for the management of cervical high-grade squamous intraepithelial lesion (HSIL) patients with positive margin by conization. Clinico-pathological data of HSIL patients with positive margin by conization were retrospectively collected from January 2009 to December 2014. All patients underwent secondary conization or hysterectomy within 6 months. The rate of residual lesion was calculated, and the factors associated with residual lesion were analyzed by univariate and multivariate analyses. Among a total of 119 patients, 56 (47.06%) patients presented residual HSIL in their subsequent surgical specimens, including 4 cases of invasive cervical carcinoma (3 stage IA1 and 1 stage IA2 patients). Univariate analysis showed that patient age>35 years (P=0.005), menopausal period>5 years (P=0.0035), and multiple-quadrant involvement (P=0.001) were significantly correlated with residual disease; however, multivariate analysis revealed that multiple-quadrant involvement (P=0.001; OR, 3.701; 95%CI, 1.496–9.154) was an independent risk factor for residual disease. Nearly half of HSIL patients with positive margin by conization were disease-free in subsequent surgical specimens, and those with multiple positive margins may consider re-conization or re-assessment.
关键词: cervical high-grade squamous intraepithelial lesion conization positive surgical margin hysterectomy
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《医学前沿(英文)》 2016年 第10卷 第1期 页码 104-110 doi: 10.1007/s11684-016-0432-4
Advances in next-generation sequencing and bioinformatics have begun to reveal the complex genetic landscape in human cancer genomes, including oral squamous cell carcinoma (OSCC). Sophisticated preclinical models that fully represent intra- and inter-tumoral heterogeneity are required to understand the molecular diversity of cancer and achieve the goal of personalized therapies. Patient-derived xenograft (PDX) models generated from human tumor samples that can retain the histological and genetic features of their donor tumors have been shown to be the preferred preclinical tool in translational cancer research compared with other conventional preclinical models. Specifically, genetically well-defined PDX models can be applied to accelerate targeted antitumor drug development and biomarker discovery. Recently, we have successfully established and characterized an OSCC PDX panel as part of our tumor bio-bank for translational cancer research. In this paper, we discuss the establishment, characterization, and preclinical applications of the PDX models. In particular, we focus on the classification and applications of the PDX models based on validated annotations, including clinicopathological features, genomic profiles, and pharmacological testing information. We also explore the translational value of this well-annotated PDX panel in the development of co-clinical trials for patient stratification and treatment optimization in the near future. Although various limitations still exist, this preclinical approach should be further tested and improved.
关键词: patient-derived xenograft models personalized medicine co-clinical trial patient stratification oral squamous cell carcinoma
功能影像检测食管癌放疗过程中再增殖和乏氧以及预测临床疗效研究
于金明
《中国工程科学》 2012年 第14卷 第7期 页码 9-19
18F-FLT PET能监测食管鳞癌放疗过程中肿瘤和正常组织的生物学变化,其较18F-FDG PET能较好区分炎症和肿瘤,能检测食管肿瘤放疗过程中的加速再增殖,18F-FETNIM PET能检测食管鳞癌的乏氧状态,18F-FLT PET和18F-FETNIM PET作为一种非创伤性的影像学技术,能为肿瘤医生提供一个早期评价治疗反应,检测放疗过程中的再增殖克隆源细胞、乏氧和评价新辅助治疗疗效的手段。18F-FLT PET和18F-FETNIM PET能为放射治疗确定“再增殖”和“乏氧”生物学靶区,以通过剂量调强放疗提高再增殖和乏氧区域放疗剂量,提高肿瘤的局部控制率和远期生存。
Microfluidics for cell-cell interactions: A review
Rui Li,Xuefei Lv,Xingjian Zhang,Omer Saeed,Yulin Deng
《化学科学与工程前沿(英文)》 2016年 第10卷 第1期 页码 90-98 doi: 10.1007/s11705-015-1550-2
关键词: microfluidic chip co-culture cell-cell interactions review
Distinct mononuclear diploid cardiac subpopulation with minimal cell–cell communications persists in
《医学前沿(英文)》 页码 939-956 doi: 10.1007/s11684-023-0987-9
关键词: mononuclear diploid cardiomyocytes cell–cell communication cardiac fibroblast single-cell RNA sequencing cardiac regeneration
Deubiquitinases as pivotal regulators of T cell functions
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《医学前沿(英文)》 2018年 第12卷 第4期 页码 451-462 doi: 10.1007/s11684-018-0651-y
T cells efficiently respond to foreign antigens to mediate immune responses against infections but are tolerant to self-tissues. Defect in T cell activation is associated with severe immune deficiencies, whereas aberrant T cell activation contributes to the pathogenesis of diverse autoimmune and inflammatory diseases. An emerging mechanism that regulates T cell activation and tolerance is ubiquitination, a reversible process of protein modification that is counter-regulated by ubiquitinating enzymes and deubiquitinases (DUBs). DUBs are isopeptidases that cleave polyubiquitin chains and remove ubiquitin from target proteins, thereby controlling the magnitude and duration of ubiquitin signaling. It is now well recognized that DUBs are crucial regulators of T cell responses and serve as potential therapeutic targets for manipulating immune responses in the treatment of immunological disorders and cancer. This review will discuss the recent progresses regarding the functions of DUBs in T cells.
关键词: deubiquitinase ubiquitination T cell activation T cell differentiation T cell tolerance
Cell surface protein engineering for high-performance whole-cell catalysts
Hajime Nakatani,Katsutoshi Hori
《化学科学与工程前沿(英文)》 2017年 第11卷 第1期 页码 46-57 doi: 10.1007/s11705-017-1609-3
关键词: cell surface engineering surface display whole-cell catalysts bioprocess
标题 作者 时间 类型 操作
Repurposed benzydamine targeting CDK2 suppresses the growth of esophageal squamous cell carcinoma
期刊论文
Relative expression of PTTG and bFGF in oral squamous cell carcinoma and Tca8113
Yumei DING BM , Lili CHEN MD , Bo CHENG PhD , Handong ZHANG MM ,
期刊论文
p53 functional activation is independent of its genotype in five esophageal squamous cell carcinoma cell
Junfang JI, Kun WU, Min WU, Qimin ZHAN,
期刊论文
GDF15 negatively regulates chemosensitivity via TGFBR2-AKT pathway-dependent metabolism in esophageal squamouscell carcinoma
期刊论文
U-shaped association between telomere length and esophageal squamous cell carcinoma risk: a case-control
null
期刊论文
Bevacizumab in combination with pemetrexed and platinum for elderly patients with advanced non-squamousnon-small-cell lung cancer: a retrospective analysis
期刊论文
Triage for management of cervical high-grade squamous intraepithelial lesion patients with positive margin
null
期刊论文
Patient-derived xenograft platform of OSCC: a renewable human bio-bank for preclinical cancer research and a new co-clinical model for treatment optimization
null
期刊论文
Microfluidics for cell-cell interactions: A review
Rui Li,Xuefei Lv,Xingjian Zhang,Omer Saeed,Yulin Deng
期刊论文
Distinct mononuclear diploid cardiac subpopulation with minimal cell–cell communications persists in
期刊论文